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Abstract
The objective of this research was to evaluate the therapeutic efficacy of an antikeratinophilic cream formulated using an extract derived from Chromolena odorata leaves (COL). The collection of garden soil samples was conducted in a random manner, and subsequent analysis included the screening for the presence of keratinophilic fungus using microbiological methods that were deemed acceptable for this purpose. The evaluation of the pathogenic potentials of the isolates was conducted utilizing an in vivo methodology. An in vivo approach was used, using albino Wistar rats, to assess the therapeutic efficacy of the produced cream against keratinophilic fungal infections.The data obtained from the research were subjected to statistical analysis using Analysis of Variance (ANOVA), Chi square (X2), and student 't' test at a confidence level of 95%. The findings of the study revealed that the soil samples included Microscoporum species (MS), Trichophyton species (TRS), and Trichophyton species (TS).The isolates demonstrated statistically significant (P < 0.05). Pathological lesions, with the highest incidence seen in the rats infected with TS. The cream that was made had a smooth and thick consistency, as shown by its viscosity of 46.70 mPa.S. It also possessed specific gravity, refractive index, and pH values of 0.904, 1.460, and 7.30, respectively. Furthermore, the cream demonstrated absence of microbial load, as evidenced by zero counts in all examined parameters. The cream exhibited notable therapeutic efficacy against the infected tissues, with the highest level of resolution seen during a time-frame of 42 to 56 days. This result was statistically significant (P < 0.05), particularly in relation to the infection induced by MS. The research findings indicate that the experimental rats had different pathological lesions when exposed to MS, TRS, and TS. However, these lesions were effectively cured utilizing a cream formulated from COL. Hence, the findings of this investigation propose that the formulated cream has potential as a viable treatment option for treating topical infections induced by keratinophilic fungus. Keywords: Keratinophilic,Chromolena odorata,Pathological,Therapeutic,Fungi.