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Abstract
The study was carried to investigate the total glutathione level and total protein of Madonna university students, Elele campus of Rivers State. Elele is a town in Rivers state which is located at the tropics of south-south of Nigeria.The population of the study consists of 40 students within the ages of 16-27 years, both male and female. The questionnaires for this study were distributed to individuals to be filled base on gender, age, active and less active individuals, drug and non-drug dependent individual and individual in different sporting activities. Blood was collected by venipuncture and serum prepared by centrifugation, serum was collected using micropipette and stored into aliquot at -20C in a fridge. Ellman reagent was used for glutathione determination while Biuret reagent was used for protein assay using biochemical standard. The results showed that the male students have higher level of glutathione concentration (0.0277 ± 0.001) when compared to female students (0.0259 ± 0.009). However, there was no significant difference (P˃0.05) in glutathione concentration of male and female students. The result also showed that female students have higher level of total protein ( 5.21 ± 0.56) when compared to the male students(5.02 ± 0.87) .Therefore, there was no significant different (P˃0.05). The total protein and glutathione level were not age dependent, there was no significant difference(P˃0.05) in glutathione level and total protein of individuals aged 16- 21 years when compared to those aged 22–27 years. Table 3.3 showed that the glutathione level and total protein were not activity dependent. In conclusion, the individuals have their total glutathione and total protein level within the normal range. These showed that the individuals are free from some of the diseases caused by the accumulation of free radical and foreign substances in the body because glutathione is an antioxidant. Keyword: Glutathione level, Antioxiant, Protein, Serum, determination, Tripeptide compound.